Regulatory

Part:BBa_K4633004:Design

Designed by: Neta Segal   Group: iGEM23_Technion-Israel   (2023-09-07)


paraR - promoter for the araR gene from Bacillus subtilis 168


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The paraR promoter, along with the native ribosome binding site (RBS) found in BBa_K4633005, was tested in Bacillus subtilis 168 as a part of the synthetic construct. This composite part was used to investigate the promoter's regulatory characteristics and its impact on downstream gene expression.

Source

The sequence of the paraR promoter was sourced from the work of Sá-Nogueira and Mota (1997) [1].

References

[1] Sá-Nogueira, I. & Mota, L. J. Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene. J. Bacteriol. 179, 1598–1608 (1997).